Comparison of the Vitek 2, API 20A, and 16s rRNA Gene Sequencing for the Identification of Anaerobic Bacteria.
- Author:
Gyun Cheol PARK
1
;
Sook Jin JANG
;
Min Jung LEE
;
Joong Ki KOOK
;
Min Jung KIM
;
Young Sook KIM
;
Nam Woong YANG
;
Hye Soo LEE
;
Seong Ho KANG
;
Geon PARK
;
Dae Soo MOON
Author Information
- Publication Type:Original Article
- Keywords: Anaerobic bacteria; API 20A; Comparison; Vitek 2; 16s rRNA Gene sequence
- MeSH: Bacteria, Anaerobic*; Genes, rRNA*
- From:Annals of Clinical Microbiology 2015;18(1):20-26
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: Recently, genotypic identification of anaerobes is emerging as an alternative to the phenotypic method. In this study, we evaluated the performance of Vitek 2, API 20A and 16s rRNA gene sequencing for the identification of anaerobic bacteria. METHODS: A total of 35 anaerobe reference strains were identified using Vitek 2, API 20A and 16s rRNA gene sequencing. We evaluated the performance of three methods on the basis of the accurate identification rates. RESULTS: The Vitek 2, API 20A and 16s rRNA gene sequencing identified 54.3, 15.4, and 94.3% of test strains correctly at the species level and identified 77.1, 42.3, and 100% at the genus level, respectively. Results of the McNemar's test showed that there was a significant difference between each of the three identification methods in species level identification (P value<0.05). CONCLUSION: 16s rRNA gene sequencing showed better performance than Vitek 2 or API 20A for anaerobic bacteria. Considering its excellent performance, 16s rRNA gene sequencing may be useful for accurate identification of anaerobic bacteria that cannot be correctly identified by phenotypic methods.
