Increased myocardial expression of calcium-sensing receptor and apoptosis in a rat model of atherosclerosis
10.3321/j.issn:0253-3758.2008.12.012
- VernacularTitle:动脉粥样硬化对大鼠心肌钙敏感受体表达和细胞凋亡的影响
- Author:
Jin GUO
1
;
Chang-Qing XU
;
Hong-Zhu LI
;
Li-Na WANG
;
Lu-Chuan WANG
;
Li ZHANG
;
Wei-Hua ZHANG
;
Guang-Wei LI
;
Ye TIAN
Author Information
1. 哈尔滨医科大学
- Keywords:
Atherosclerosis;
Receptors,calcium-sensing;
Myocardium;
Apoptosis
- From:
Chinese Journal of Cardiology
2008;36(12):1101-1105
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of hyperlipidemia and atheroselerosis on rat myocardial expression of calcium-sensing receptor and alyJptosis.Methods The rat atherosclerosis model was induced by intraperitoneal injection of VD3(6×105U/kg)and high cholesterol diet.Wistar rats were divided into two groups:①Control group;②AS group(n=12 each).The expressions of CaSR,Bcl-2,Bax and caspase-3 were analyzed by Western blot and RT-PCR.Apoptotic cells were observed by TUNEL assay.The morphological changes of abdominal aorta and cardiac tissues were observed under optical and electro microscopes.The activity of LDH,CK,SOD and the content of MDA were assayed with ultraviolet spectrophotometer.The level of cTnT waft detected by electrochemical immunofluoresence.Results Compared with control group,the activity of LDH and CK,the content of M DA and cTnT,the apoptosis index,the expression of CaSR,Bax and caspase-3 were significantly increased,but the SOD activity and Bcl-2 expression were significantly decreased,the myocardial uhrastructure injury was significantly aggravated in the AS group(all P<0.05).Conclusion Hyperlipidemia and atherosclerosis can up-regulate myocardial calcium-sensing receptor expression,promote myocardial apoptosis,aggravate oxidative stress and myocardial ischemia.
