The effect of oxidized low-density lipoprotein on the maturation and its immune function of monocyte-derived dendritic cells
10.3321/j.issn:0253-3758.2008.12.013
- VernacularTitle:氧化型低密度脂蛋白对外周血单核来源树突状细胞成熟及免疫功能的影响
- Author:
Hang WANG
1
;
Hao HUANG
;
Ke-Yin CAI
Author Information
1. 广州军区武汉总医院
- Keywords:
Athemsclerosis;
Lipoproteins,LDL;
Dendritic ceils;
NF-kappa B
- From:
Chinese Journal of Cardiology
2008;36(12):1106-1109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of ox-LDL on monocle-derived dendritic cells.Methods DCs were derived from healthy donors and divided into four groups according to the method of stimulation.The cells of blank group,negative group,experimental group and positive group which were treaded with PBS,LDL,ox-LDL,TNF-a,individually.ox-LDL was added during the late stage of monocyte differentiation. Flow cytometry was used to analyze the cell surface markers and the endocytes of DCs.3H-TdR incorporation was used to measure the proliferation of syngeneie and allogeneic T cells.ELISA assay was used to measure IL-12,MCP-1 and MIP1 in cultured medium.Western blot analysis Was used to evaluate the content of IKBa and NF.KB of DCs.Resuits Addition of ox-LDL during the late stage of monocytes differentiation can upregulate the cell surface markers including CD40(22.3% vs.45.6%)and CD86 (25.9%VS.82.4%),increase the secretion of IL-12(31.43 pg/ml vs.126.73 pg/ml)and MCP-1(59.6 ng/ml vs.116.3 ng/ml),reduce DCs uptake capacity(46.8% vs.10.7%),enhance allogeneic T cells proliferation(SI:4.5 vs.5.7),promote IkBa degradation and upregulate the expression of NF-kB in DCs.Conclusion ox-LDL can promote the maturation of PBMCs-derived DCs by promoting IkBa degradation.
