Evaluation of the Performance of a New Chromatographic Assay BD Veritor System for Rapid Detection of Influenza A & B.
- Author:
Sholhui PARK
1
;
Miae LEE
;
Hae Sun CHUNG
Author Information
- Publication Type:Original Article
- Keywords: Influenza; Rapid influenza diagnostic test; Real-time RT-PCR; Sensitivity
- MeSH: Biological Science Disciplines; Diagnosis; Diagnostic Tests, Routine; Early Diagnosis; Humans; Influenza B virus; Influenza, Human*; Mass Screening; Orthomyxoviridae; Pathology, Molecular; Polymerase Chain Reaction; Real-Time Polymerase Chain Reaction; Sensitivity and Specificity
- From:Annals of Clinical Microbiology 2015;18(1):27-32
- CountryRepublic of Korea
- Language:Korean
- Abstract: BACKGROUND: For early diagnosis and treatment of influenza, rapid influenza diagnostic tests are commonly used. We evaluated the performance of the BD Veritor System for Rapid Detection of Flu A+B (BD Veritor System; BD Diagnostics, USA) compared to multiplex real-time RT-PCR. METHODS: A total of 3,213 nasal and nasopharyngeal swab specimens in transport media from patients with influenza-like symptoms were tested with the BD Veritor System from December 2013 to April 2014. The sensitivity and specificity of 127 specimens were determined simultaneously using multiplex real-time RT- PCR with the AdvanSure RV real-time PCR (AdvanSure PCR; LG Life Sciences, Korea). RESULTS: Influenza viruses were detected in 41.3% (1,327/3,213) of all specimens tested using the BD Veritor System. Of the 127 specimens, 27 influenza A and 17 influenza B viruses were identified by the AvanSure PCR. The sensitivity and specificity of the BD Veritor System relative to the AdvanSure PCR was 85.2% and 99.0% for influenza A, and 58.8% and 99.1% for influenza B. Of the 190 specimens that tested negative using the BD Veritor System, the AdvanSure PCR detected influenza A and influenza B in 19 and 13 specimens, respectively. The mean threshold cycle (Ct) values of the antigen positive specimens were lower than those of the antigen negative specimens. CONCLUSION: The BD Veritor System showed excellent specificity for both influenza types and good sensitivity for influenza A. However, the system was less sensitive for influenza B compared to multiplex real-time RT-PCR. For accurate diagnosis of false negative specimens, a molecular diagnostic test should be performed. The BD Veritor system could be a useful tool for screening and early diagnosis of influenza.
