Sinoatrial node cells phenotype expression is induced by direct bone marrow stem cells contact with sinoatrial node cells

VernacularTitle:细胞接触诱导骨髓间充质干细胞获得窦房结细胞表型的初步研究
Author: Si-Bin GUAN ¹ ; Ai-Qun MA ; Wen-Hui JIANG
Author Information

1. 上海徐汇区中心医院
Keywords: Stem cells; Sinoatrial node
From: Chinese Journal of Cardiology 2009;37(1):73-76
CountryChina
Language:Chinese
Abstract: Objective To observe the differentiation of bone mesenehymal stem cells(BMSCs)cocultured with purified sinoatrial node cells(SNC)of neonate rata.Methods SNC from neonatal SD rat were cuhured and Durifted with differential attachment method and labded with BrdU.Rat BMSCs Were isolated by a Percoll's gradient solution and cultared in DMEM.After 2 passages,these BMSCs were transfected with pEGFP-N1 by Lipofectamin and labeled with GFP.EGFP-BMSC were co-cultured with SNC in a rate of 1:5 for 1 week.EGFP-BMSC cultured in SNC culture medium served as controls.SNC marker hyperpolarization activated cyclic nueleotide gated cation channel 4(HCN4) and connexin 45 (Cx45) expressions were determined by inunanofluorescence staining.Result Positive immunonuorescence staining against HCN4 and Cx45 were detected in EGFP-BMSC co-cultured with SNC but not in EGFP-BMSC cultured in SNC cuhure medium.Conclusion Direct cell-to-cell contact between BMSCs and SNC ceils may induce BMSCs differentiation into sinus node-like cells.