Influence of cyclosporine A on atrial L-type calcium channel αlc subunit in a canine model of atrial fibrillation
10.3760/cma.j.issn.0253-3758.2009.02.005
- VernacularTitle:环孢素A对心房颤动犬心房L型钙离子通道蛋白αlc亚单位的影响
- Author:
Ya HUANG
1
;
Cai-Yi LU
;
Wei YAN
;
Lei GAO
;
Qi CHEN
;
Ya-Jing ZHANG
Author Information
1. 解放军总医院
- Keywords:
Atrial fibrillation;
Calcium channels;
Cyclosporine A
- From:
Chinese Journal of Cardiology
2009;37(2):112-114
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of cyclosporine A (CsA) on atrial expression change of L-type calcium channel αlc subunit in a canine model of atrial fibrillation (AF). Methods AF was induced by rapid atrial pacing (400 baets/min) for 8 weeks in adult male dogs and placebo (n=6) or CsA (5 mg·kg-1·d-1, n=6) were orally administered to these animals. Sham operated animals served as normal controls (n=6). The atrial electrophysiological parameters including P wave duration, atrial effective refractory period (AERP) were recorded and analyzed at baseline and 8 weeks later. Animals were scarified at 8 weeks post final electrophysiological examinations and atrial expressions of L-type calcium channel αlc subunit were determined by Western blot. Results Compared to sham group, the P wave duration was significantly prolonged while AERP was significantly decreased in AF and CsA groups (all P <0. 05). AERP was significantly longer in CsA group than that in AF group (P < 0. 05 ). L-type calcium channel αlc subunit expression was significantly downregulated in AF group compared to sham group (P <0. 05) and CsA significantly attenuated this downregulation (P < 0. 01 vs. AF group). Conclusion CsA could attenuate the downreguahion of the L-type calcium channel αlc subunit expression and improve the atrial electrophysiological remodeling in this canine model of AF.
