Identification of differentially expressed genes in myocardium of patients with heart failure by human whole genomic oligonucleotide microarray-assisted pathways analysis
10.3760/cma.j.issn.0253-3758.2009.02.007
- VernacularTitle:全基因组芯片结合通路分析筛选心力衰竭患者心肌细胞差异基因
- Author:
Xiao-Xia WU
1
;
Tao WAN
;
Hong-Jin WU
;
Guang ZHI
;
Cang-Song XIAO
;
Chang-Qing GAO
;
Jia-Jin WU
Author Information
1. 武警总医院
- Keywords:
Heart failure,congestive;
Gone expression;
Signal transduction
- From:
Chinese Journal of Cardiology
2009;37(2):120-125
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the differentially expressed gene profiles in myocardium of patients with heart failure using human whole genomic oligonucleotide microarray-assisted pathway analysis. Methods Phalanx whole genomic oligonucleotide microarrays were used to detect the gene expression profiles of myocardium in four patients died of heart failure and 4 brain died patients without heart diseases. The microarray findings were confirmed by real-time quantitative reverse transcriptase-polymerase chain reaction. The genes with a threshold of 1.2 times fold-change were selected and BioCarta Pathway and KEGG ( Kyoto Encyclopaedia of Genes and Genomes) pathway databases were used to identify functionally related gene pathways. Results A total of 2806 genes with differentially expression were detected between the failing and non-failing heart samples, expression changes of 399 genes were more than 2-folds. Eleven pathways were identified by BioCarta pathway database and sixteen athways were identified by KEGG PATHWAY Database. Conclusion Genomic microarray-assisted pathway analysis could help to identify gene expression profiles in failing heart.
