Hydrogen sulfide induce negative inotropic effect in isolated hearts via KATP channel and mitochondria membrane KATP channel
10.3760/cma.j.issn.0253-3758.2009.02.018
- VernacularTitle:线粒体膜钾通道阻断剂在硫化氢对大鼠心功能调节的意义
- Author:
Yan SUN
1
;
Su-Qing ZHANG
;
Hong-Fang JIN
;
Chao-Shu TANG
;
Jun-Bao DU
Author Information
1. 北京大学第一医院
- Keywords:
Hydrogen sulfide;
Potassium channels;
Ventricular function,left
- From:
Chinese Journal of Cardiology
2009;37(2):161-164
- CountryChina
- Language:Chinese
-
Abstract:
Objective Hydrogen sulfide (H2S) dilates blood vessels in vivo and in vitro probably by opening vascular smooth muscle K+-ATP channels. The study was designed to observe the role of mitochondria membrane KATP channel blocker (5-HD)in the regulation of cardiac function isolated perfused heart of rat with H2S. Methods The isolated rat heart was perfused in a Langendorff apparatus. After 20 minutes of stabilization, physiological concentration of NaHS (H2S donor, 100 μmol/L) was continuously perfused for 20 min in group A (n=6), isolated hearts in group B (n=6 ) and C (n=7) were pretreated with nonspecific KATP channel blocker glibenclamide (100 μmol/L) or 5-HD (100 μmol/L) for 5 minutes then perfused with NaHS (100 μmol/L) for 10 minutes. Heart rate (HR), left ventricular developed pressure (△LVP), dp/dtmax and dp/dtmax and coronary perfusion flow (CPF) were measured. Results Post continuous perfusion of NaHS at physiological concentration for 20 minutes, △LVP, dp/dtmax and dp/dtmax all significantly decreased while HR and CPF remained unchanged compared to baseline levels (all P<0.05). The negative inotropie effect of H2S could partly be blocked by nonspecific KATP channel blocker glibenclamide and mitochondria membrane KATP channel blocker 5-HD. Conclusion Present findings suggested that H2S at physiological concentration could produce negative inotropic effect in isolated hearts and this effect was mediated by KATP channel and mitochondria membrane KATP channel.
