Ad-TFPI gene transfer attenuates intimal proliferation in rabbit carotid arteries after balloon injury
10.3760/cma.j.issn.0253-3758.2009.02.020
- VernacularTitle:腺病毒介导组织因子途径抑制物基因转染兔损伤颈动脉后的表达及对内膜增生的抑制作用
- Author:
Yan-An ZOU
1
;
Jing-Bo HOU
;
Yao ZHANG
;
Hong-Gang NIE
;
Hai-Xia LIU
;
Bo YU
Author Information
1. 哈尔滨医科大学附属第二医院
- Keywords:
Tunical intimal;
Hyperplasia;
Genes;
Therapy;
Tissue factor pathway inhibitor
- From:
Chinese Journal of Cardiology
2009;37(2):169-173
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vivo gene expression of adenovirus-mediated human tissue factor pathway inhibitor (hTFPI) and its inhibition effects on intimal proliferation in rabbit carotid arteries after ballon injury. Methods Rabbits underwent carotid artery balloon injuries were treated with Ad-TFPI (n=25), Ad-LacZ (n=25) or PBS (n=10), respectively. Sham operated rabbits (n=10) serve as normal controls. The expressions of human TFPI at mRNA and protein levels were detected by RT-PCR and ELISA respectively on the 3rd, 7th, 10th, 14th, 28th day after operation. Intimal proliferation was detected by angiograms and morphometric analysis. Results TFPI mRNA and protein expressions were detected at 3 days and peaked at the 10th and 14th day after TFPI gene transfer. The expressions were still detectable on the 28th day. There was no TFPI expression in Ad-LacZ group. The carotid angiogram results indicated that the minimal lumen diameter in TFPI group was significantly larger and the lumina stenosis percentage was significantly lower in TFPI group compared those in Ad-LacZ and PBS groups (all P < 0.05). The morphometric analysis showed that the intimal area, the ratio of the intimal/media area, the lumina stenosis percentage in TFPI group were all significantly reduced compared with those in Ad-LacZ and PBS groups (all P<0.01).Conclusions The TFPI gene could be effectively transferred by adenovirus vector to injured carotid arteries in rabbits.
