Effect of a novel injectable hydrogel implant on ventricular remodeling in rabbits with experimental myocardial infarction
10.3760/cma.j.issn.0253-3758.2009.02.022
- VernacularTitle:新型可注射水凝胶植入对兔心肌梗死后心室重构的影响
- Author:
Tao WANG
1
;
Xue-Jun JIANG
;
Tao LIN
;
Xiao-Yan LI
;
Jin-Feng ZHANG
;
Zhao-Bin ZHENG
;
Jin-Ling CHEN
Author Information
1. 武汉大学人民医院
- Keywords:
Myocardial infarction;
Biocompatible materials;
Ventricular remodeling;
Tissue
- From:
Chinese Journal of Cardiology
2009;37(2):174-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe whether injectable hydrogel implantation could prevent adverse cardiac remodeling and preserve cardiac function in rabbits with myocardial infarction (MI). Methods A novel injectable hydrogel, the copolymer MPM/α-CD, which self-assembled between α-cyclodextrin and methoxy polyethylene glycol-poly (caprolactone)-(dodecanedioic acid)-poly (caprolactone)-methoxypolyethylene glycol triblock polymer, was synthesized by chemical crosslinking and characterized by biocompatibility and biodegradability. Experimental MI was induced in male rabbits by coronary artery ligation. The MI rabbits were randomly divided into hydrogel group (200 μl MPM/α-CD were injected into the infarcted myocardium 7 days after MI) and control group (equal volume phosphate buffered saline myocardial injection, n=8 each). Four weeks after MPM/α-CD implantation, echocardiography,histochemistry and immunohistochemistry were performed. Results Left ventricle ejection fraction was significantly improved in the hydrogel-treated group (56.1% ± 8.4%) than that in the control group (37.3% ±6.4%, P <0.05). Histological analysis indicated that hydrogel degraded 4 weeks after hydrogel injection, and prevented scar expansion and wall thinning [(3.08 ± 0.32) mm vs. (2.18 ± 0.46) mm in control group, P <0.05]. Neovasculature formation was similar between the hydrogel group [(100.8 ± 2.4)/mm2] and the control [(98.5 ± 2.9)/mm2, P>0.05]. onclusion MPM/α-CD could serve as an excellent injectable biomaterial for improves cardiac function and attenuating scar expansion and left ventricular dilation in MI rabbits.
