Gelatin microspheres containing vascular endothelial growth factor enhances the efficacy of bone marrow mesenchymal stem cells transplantation in a swine model of myocardial infarction
10.3760/cma.j.issn.0253-3758.2009.03.008
- VernacularTitle:细胞因子微球加强骨髓间充质干细胞移植对猪心肌梗死的疗效
- Author:
Qiong LIU
1
;
Shi-Hua ZHAO
;
Min-Jie LU
;
Shi-Liang JIANG
;
Chao-Wu YAN
;
Yan ZHANG
;
Liang MENG
;
Yue TANG
;
Xian-Min MENG
;
Ying-Jie WEI
;
Lin-Lin WANG
;
Hong-Jun DAI
;
Jian XU
Author Information
1. 中国医学科学院北京协和医学院阜外心血管病医院
- Keywords:
Myocardial infarction;
Magnetic resonance imaging;
Stem cell transplantation
- From:
Chinese Journal of Cardiology
2009;37(3):233-239
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the efficacy of transplantation of mesenchymal stem cells(MSC) with gelatin micrespheres containing vascular endothelial growth factor in ischemie regions in infracted swine hearts. Methods Twelve Chinese mini swines with infarction were randomized to receive autogenetic MSC injection to the peri-infarction area of left ventricular wall (MSC group, n=6) or MSC transplantation with gelatin hydrogel microspheres incorporating vascular endothelial growth factor (VEGF-MSC group, n=6). Three weeks later, left ventricular function was assessed by magnetic resonance imaging (MRI). The contrast of the MSC hypointense lesion was determined using the difference in signal intensity between the hypointenso and normal myocardium divided by signal intensity of the normal region. Myocardial capillary density, the number of DAPI positive MSC and the apoptotic MSC were also determined. Results The diameter of the microspheres averaged (104.0±22.6) μm. At 24 hours after transplantation, MSC were identified by MRI as large intramyocardial signal voids at injection sites which persisted up to 3 weeks. There was no significant difference in the contrast of the lesions and in the size of the lesions at 24 hours between two groups. At 3 weeks after injection, the size of the lesions and the contrast of the lesion were decreased (P<0.05) in both groups. The capillary density of the injection site was significantly more in the MSC-VEGF microsphere group than that in MSC group [ (15.2±5.4)/HPF vs. (10.2±5.0)/HPF, t=2.43, P<0.05], and there were more dense DAPI labeled MSC per high power fields in injection sites of MSC-VEGF mieresphere group than that in MSC group [(354±83)/HPF vs. (278±97)/HPF, t=3.14, P<0.05]. Moreover, the apoptosis rate of MSCs of MSCs-VEGF mieresphere group was less than that of MSCgroup [(6.4±4.1)% vs. (11.9±4.8)%, t=2.97, P<0.05]. Conclusions MSC transplantation with gelatin hydrogel microspheres incorporating VEGF enhanced the efficacy of MSC in this swine model of myocardial infarction. MRI tracking of MSC is feasible and represents a preferred method for studying the engraftment of MSCs in infracted tissue.
