Effects of hydrogen sulfide on the KATP current in isolated rat ventricular myocytes

VernacularTitle:硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响
Author: Yan-Bing LI ¹ ; Guang-Zhen ZHONG ; Xin-Chun YANG ; Xiu-Lan LIU
Author Information

1. 首都医科大学附属北京朝阳医院
Keywords: Hydrogen sulfide; Potassium channels; Patch-clamp techniques
From: Chinese Journal of Cardiology 2009;37(5):445-448
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of endogenous and exogenous hydrogen sulfide (H2S) on the KATP current in isolated rat ventricular myoeytes. Methods Ventrieular myoeytes were isolated from rat heart by modified Langendoff perfusion with collagenase. KATP current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. Results The density of KATP current was significantly reduced by 200 μmol/L DL-propargylglyeine (PPG, an irreversible inhibitor of the H2S) [(5.3258±0.7556) pA/pF vs. (3.7856±0.4312) pA/pF, P ＜ 0.01] in a time-dependent way. The density of KATP current could be significantly increased by NariS(a H2S donor, 9.375, 18.75, 37.5,75, 150 μmol/L) in a concentration-dependent manner [(6.6310±0.6092) pA/pF vs. (9.0949±1.0259)pA/pF at 150 μmol/L, P ＜ 0.01]. Conclusion Both endogenous and exogenous H2S could open KATP channels and enhance the KATP current in rat ventricular myocytes.