Effect of lipopolysaccharide on the stromal cell-derived factor-1 expression in human stem cells from apical papilla.
- Author:
Jingyi LIU
1
;
Xiaoying ZOU
1
;
Xue CHEN
1
;
Ye CHEN
1
;
Lin YUE
2
;
Email: KQLINYUE@BJMU.EDU.CN.
Author Information
- Publication Type:Journal Article
- MeSH: Cell Differentiation; Cell Proliferation; drug effects; Chemokine CXCL12; genetics; metabolism; Dental Papilla; cytology; Gene Expression; Humans; Lipopolysaccharides; pharmacology; Stem Cells; drug effects; metabolism
- From: Chinese Journal of Stomatology 2015;50(6):346-351
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of stromal cell-derived factor-1 (SDF-1) in human stem cells from apical papilla (SCAP), and to evaluate the effect of lipopolysaccharide (LPS) on SDF-1 expression by SCAP.
METHODSSCAP were isolated from dental papilla of human immature third molars. The expression of SDF-1 was evaluated by reverse transcription-PCR (RT-PCR). After SCAP being exposed to different concentrations (0.1, 1.0, 10 mg/L) of LPS for 24 and 48 h, the effect of LPS on cell proliferation and gene expression of SDF-1 was investigated by cell counting kit-8 and real-time PCR respectively, while cells without LPS stimulation were considered as negative control.
RESULTSLPS had no significant effect on SCAP proliferation until day 7. RT-PCR assays demonstrated that SCAP expressed SDF-1 mRNA. Different concentrations of LPS significantly promoted the SDF-1 expression in SCAP after 24 h (F = 12.102, P = 0.002) and 48 h (F = 39.054, P < 0.001) exposure, with relative gene expression ratio (experimental/control) increased to 1.4 ± 0.1, 2.2 ± 0.4, 2.3 ± 0.5 in 24 h group and 2.1 ± 0.4, 3.4 ± 0.3, 3.8 ± 0.5 in 48 h group.
CONCLUSIONSIsolated SCAP in cultures have the expression of SDF-1 mRNA. LPS can significantly promote the expression of SDF-1 in SCAP.
