Study of oral microbial adhesion and biofilm formation on the surface of nano-fluorohydroxyapatite/polyetheretherketone composite
10.3760/cma.j.issn.1002-0098.2015.06.013
- VernacularTitle:含氟纳米羟基磷灰石-聚醚醚酮复合材料表面变形链球菌黏附和生物膜形成初探
- Author:
Lixin WANG
1
;
Huan ZHANG
;
Yi DENG
;
Zuyuan LUO
;
Xiyun LIU
;
Shicheng WEI
Author Information
1. 100038,首都医科大学附属北京世纪坛医院口腔科
- Keywords:
Hydroxyapatites;
Fluorides;
Biofilms;
Polyetheretherketone
- From:
Chinese Journal of Stomatology
2015;50(6):378-382
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop novel polyetheretherketone(PEEK) based nanocomposites which possess the favorable antibacterial property,and to investigate the oral microbial adhesion and biofilm formation on the surfaces of PEEK,nano-fluorohydroxyapatite(n-FHA)-PEEK and nano-hydroxyaptite (n-HA)-PEEK.Methods The bacterial adhesion and biofilm formation on the surfaces of n-FHA-PEEK,n-HA-PEEK were investigated via microbial viability assay kit and laser scanning confocal microscope (LSCM),respectively,with pure PEEK as control group.Results No significantly statistical difference were found in the bacterial adhesion amounts on the surfaces of n-FHA-PEEK,n-HA-PEEK and PEEK at 1 h and 4 h.However,the number of bacteria on the n-FHA-PEEK surface decreased dramatically at 2 h(0.496±0.008) compared with n-HA-PEEK groups(0.543± 0.015,P＜0.01).Although the biofilms formation on surfaces observed by LSCM had similar morphology and thickness at 3,7,14 d,that on the n-FHA-PEEK surface showed the highest dead-to-live bacteria ratio among the three materials at 14 d.Conclusions The combination of n-HA,especially for the n-FHA could inhibit the bacteria adhesion and accelerate the bacterial death,eventually may have an influence on the structure of biofilms and reduce the risk of periimplantitis.Therefore,n-FHA-PEEK nanocomposites presented a good prospect for clinical applications as dental implant materials.
