The influence of zoledronic acid on vascular endothelial cell
10.3760/cma.j.issn.1002-0098.2015.07.004
- VernacularTitle:唑来膦酸对血管内皮细胞损伤的影响
- Author:
Miaojie LANG
1
;
Zhihui ZHOU
;
Jingjing MAO
;
Manman REN
;
Li ZHU
;
Yanliang WANG
Author Information
1. 温州医科大学口腔医学院·附属口腔医院口腔颌面外科
- Keywords:
Diphosphonates;
Osteonecrosis;
Vascular endothelial
- From:
Chinese Journal of Stomatology
2015;50(7):399-402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of zoledronic acid on vascular endothelial cells.Methods The influence of zoledronic acid on proliferation,migration and adhesion of vascular endothelial cells were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT),cell migration assay and cell adhesion assay.The results of each experimental group were compared with the control group and the data statistically analyzed.Results In a concentration of 0-0.5 mmol/L,the absorbance value decreased from 0.09 to 0.34 as the drug concentration increased.Scratch test showed that the change of width of scratch before and after 24 hours in control,low,medium and high concentration groups were (38.7± 0.42),(35.8 ± 4.17),(19.9 ± 0.57) mm(P＜0.001),(12.5 ± 3.89) mm(P＜0.05).Adhesion test showed that the absorbance value in control,low,medium and high concentration groups were 1.14 ±0.18,0.95±0.13,0.81 ± 0.11 (P＜0.01),0.67 ±0.19(P＜0.001).Comparisons between control and experimental groups were analyzed by ttest and P values＜ 0.05 were considered statistically significant.Conclusions Zoledronic acid inhibits the proliferation,migration and adhesion of vascular endothelial cells.
