Antibacterial effect of the carboxymethyl chitosan zinc peptide on several periodontal pathogens in vitro.
- Author:
Jia SONG
1
;
Jing DENG
2
;
Yuanyuan WU
;
Wanshun LIU
;
Fulai SONG
Author Information
- Publication Type:Journal Article
- MeSH: Aggregatibacter actinomycetemcomitans; drug effects; Anti-Bacterial Agents; pharmacology; Anti-Infective Agents; Chitosan; analogs & derivatives; pharmacology; Colony Count, Microbial; Microbial Sensitivity Tests; Porphyromonas gingivalis; drug effects; Prevotella intermedia; drug effects; Zinc
- From: Chinese Journal of Stomatology 2015;50(7):413-417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the antimicrobial effects of carboxymethyl chitosan zinc (CMC-Zn(+)) and CMC-Zn(+)-peptide (CMC-Zn(+)-P) on four kinds of periodontal pathogens.
METHODSDilution method was used to determine the minimum inhibitory concentration (MIC) of CMC-Zn(+) for Porphyromonas gingivalis (Pg), Actinobacillusactinomycetemcomitans (Aa), Prevotella intermedia (Pi) and Actinomyces viscosus (Av). The antimicrobial characters of CMC-Zn(+) and CMC-Zn(+)-P on these four kinds of pathogens were evaluated by disk diffusion method.
RESULTSThe MIC of CMC-Zn(+) for Pg, Aa, Pi and Av was 0.312 5%, 0.156 25%, 0.156 25% and 0.078 125% respectively. Significant antimicrobial effects were improved along with the increased concentration of CMC-Zn(+) and CMC-Zn(+)-P (P < 0.01), while the best antimicrobial concentrations of both CMC-Zn(+) and CMC-Zn(+)-P were 5%. The antimicrobial effect of CMC-Zn(+)-P was better than that of CMC-Zn(+) on the same kind of periodontal pathogen (P < 0.01). CMC-Zn(+)-P showed different antimicrobial effects on the four periodontal pathogens (Av > Aa > Pi or Av > Aa > Pg, P < 0.01).
CONCLUSIONSCMC-Zn(+) and CMC-Zn(+)-P have inhibition effect on Pg, Aa, Pi and Av, and the best antibacterial concentration was 5%. The CMC-Zn(+)-P has better antibacterial effect than CMC-Zn(+) on Pg, Aa, Pi and Av.
