Antibacterial effect of the carboxymethyl chitosan zinc peptide on several periodontal pathogens in vitro
10.3760/cma.j.issn.1002-0098.2015.07.007
- VernacularTitle:羧甲基壳聚糖锌多肽复合材料对四种牙周致病菌体外抑菌作用的研究
- Author:
Jia SONG
1
;
Jing DENG
;
Yuanyuan WU
;
Wanshun LIU
;
Fulai SONG
Author Information
1. 青岛大学附属医院口腔内科
- Keywords:
Chitosan;
Zinc;
Microbial sensitivity tests;
Peptide;
Periodontal pathogens
- From:
Chinese Journal of Stomatology
2015;50(7):413-417
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the antimicrobial effects of carboxymethyl chitosan zinc (CMC-Zn +) and CMC-Zn +-peptide (CMC-Zn +-P) on four kinds of periodontal pathogens.Methods Dilution method was used to determine the minimum inhibitory concentration(MIC) of CMC-Zn + for Porphyromonas gingivalis(Pg),Actinobacillusactinomycetemcomitans(Aa),Prevotella intermedia(Pi) and Actinomyces viscosus(Av).The antimicrobial characters of CMC-Zn+ and CMC-Zn+-P on these four kinds of pathogens were evaluated by disk diffusion method.Results The MIC of CMC-Zn+ for Pg,Aa,Pi and Av was 0.312 5％,0.156 25％,0.156 25％ and 0.078 125％ respectively.Significant antimicrobial effects were improved along with the increased concentration of CMC-Zn+ and CMC-Zn+-P(P＜0.01),while the best antimicrobial concentrations of both CMC-Zn+ and CMC-Zn+-P were 5％.The antimicrobial effect of CMC-Zn+-P was better than that of CMC-Zn+ on the same kind of periodontal pathogen(P＜0.01).CMC-Zn+-P showed different antimicrobial effects on the four periodontal pathogens(Av＞Aa＞Pi or Av＞Aa＞Pg,P＜0.01).Conclusions CMC-Zn + and CMC-Zn +-P have inhibition effect on Pg,Aa,Pi and Av,and the best antibacterial concentration was 5％.The CMC-Zn+-P has better antibacterial effect than CMC-Zn+ on Pg,Aa,Pi and Av.
