Preliminary study on transdermal characteristics and sunface anesthetic effects of lidocaine hydrochloride loaded trans-activator of transcription peptide conjugated nano-niosome in animals
10.3760/cma.j.issn.1002-0098.2015.07.009
- VernacularTitle:穿膜肽纳米类脂质体包载利多卡因的透皮特性及表面麻醉效果初探
- Author:
Yue WANG
1
;
Lianyun ZHANG
;
Changyi LI
;
Hanjie WANG
;
Qin LI
Author Information
1. 300070,天津医科大学口腔医院正畸科
- Keywords:
Lidocaine;
Liposomes;
Anesthesia,dental;
Trans-activator of transcription peptide
- From:
Chinese Journal of Stomatology
2015;50(7):423-427
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare a new dental topical anesthetics,lidocaine hydrochloride loaded trans-activator of transcription peptide conjugated nano-niosome(LID-TAT-N),and to evaluate its transdermal properties and topical anesthesia effects.Methods LID-TAT-N was prepared using reversephase evaporation method,and lidocaine loaded conventional liposome(LID-CL) was prepared in the same manner as positive control.The diameter,ξ potential and encapsulation efficiency of LID-TAT-N and LID-CL were measured.The skin permeation of LID-TAT-N was examined,and compared with LID-CL and lidocaine injection(LID-IJ,as negative control),using a Franz diffusion cell mounted with depilated mouse skin in vitro for 12 hours.Each experiment was repeated six times.The anesthetic effect of the new topical anesthetic was investigated on the cornea of rabbits.Results The mean diameter of LID-TAT-N was smaller than that of LID-CL[(152.7 ± 10.6) nm vs.(259.5 ± 15.5) nm,P＜0.01].The 12 h cumulative permeation amount was significantly higher in LID-TAT-N group[(1 340.0±97.5) μg· cm-2] than those of LID-CL and LID-IJ groups[(1 060.6±80.2),(282.6±65.1) μg· cm-2,respectively,P＜0.05].Rabbit corneal reflex results showed that LID-TAT-N had anesthetic effect and the duration of analgesia[(24.8±2.8) min] was also longer than that of LID-IJ[(14.5 ± 2.3) min,P＜0.05].Conclusions LID-TAT-N had good transdermal ability,and the advanced skin penetration feature can improve its tropical anesthetic effect.
