Phage display peptide library for screening the peptides that specifically bind to osteoblast cells
10.3760/cma.j.issn.1002-0098.2015.07.012
- VernacularTitle:噬菌体肽文库筛选人成骨细胞特异性结合多肽的实验研究
- Author:
Mingyue WU
1
;
Yuqin ZHOU
;
Mingliang DU
;
Haiyan QIAN
;
Quanli LI
Author Information
1. 安徽医科大学口腔医学院·口腔医院政务区门诊部安徽省口腔疾病研究中心实验室
- Keywords:
Peptide library;
Dental implants;
Osteoblasts;
Amino acid sequence
- From:
Chinese Journal of Stomatology
2015;50(7):438-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the experimental methods that the phage peptide library technology screening human osteoblast specificity polypeptide,which will provide the basis of the experiment of the Ti surface biolization modification.Methods Human calvarial osteoblasts were used as the target cells for whole-cell biopanning from a 12-mer peptide phage-display library.Cell eluent and cell lysis buffer were cultivate and count respectively after washing.Then the target cells were analyzed by enzyme-linked immunosorbent assay(ELISA) and immunofluorescence detection to authenticate the positive phage clones by human gingival fibroblast as the absorber cells.The positive phage clones were deduced by DNA sequencing.Results After four rounds of screening,twenty-two positive phage clones were found out from randomly selected phage monoclonals,whose single-strand DNA were extracted and sequenced.Amino acid sequence of the highest frequency peptide was MGWSWWPETWPM,Conclusions The specific peptide against human osteoblasts can be obtained from a phage-display peptide library for use as a new research approach and experimental basis of the biolization modification of the titanium surface.
