Interleukin-17 expression induced by orthodontic force in periodontal ligament in different periodontal condition
10.3760/cma.j.issn.1002-0098.2015.10.011
- VernacularTitle:正畸力诱导下白介素17在大鼠不同状态牙周组织中的表达
- Author:
Li CHEN
1
;
Xiaotong LI
;
Xiaochuan FAN
;
Xiaoqing WEI
;
Hongmei WANG
Author Information
1. 100050,首都医科大学口腔医学院正畸科
- Keywords:
Interleukin-17;
Orthodontic extrusion;
Periodontitis;
Periodontium
- From:
Chinese Journal of Stomatology
2015;50(10):619-623
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate interleukin (IL)-17 expression induced by orthodontic force in periodontal ligament under different periodontal conditions.Methods Seventy-seven male SD rats were divided randomly into 5 groups:healthy movement group (HM),active periodontitis movement group (PM) recovery periodontitis movement group (RM),positive control group (PC) and normal control group (NC).Active periodontitis was set up by means of ligature+susceptible bacteria+suger water in PM,PC and RM groups.This stimulating factor was removed in RM group.NiTi spring was used to apply 0.49 N mesial force to move upper first molar of the rats in HM,PM and RM groups.HM,PM and RM group rats were sacrificed on day 3 (d3),day 7 (d7) and day 14 (d14).Expression changes of IL-17 in periodontal tissue in each group were measured using immunohistochemical stain and real time fluorescence quantitative PCR.Results IL-17 expression in periodontal ligament showed more positively in PM and RM groups compared with NC,PC and HM groups.No difference was found in the expression of IL-17 mRNA in NC (1.00±0.00) (P＞0.05),except in HM d14 (1.00±0.07) and RM d14 (1.19±0.15).Higher expression of IL-17 mRNA (P＜0.05) was found in other groups.The expression of IL-17 mRNA in RM d3 and d7 increased more than that in HM group in the same period (P＜0.05) and it decreased significantly (P＜0.05) compared with that in PM groups in the same period.Conclusions IL-17 was involved in the regulation of orthodontic tooth movement in different periodontal conditions.Orthodontic force caused limited increase of IL-17 mRNA expression in recovery phase of periodontitis at early stage,then back to normal.However,in active inflammatory periodontal tissue,orthodontic force kept IL-17 mRNA at high level.
