Effect of zoledronic acid on cell proliferation and apoptosis of human periodontal fibroblasts
10.3760/cma.j.issn.1002-0098.2015.11.006
- VernacularTitle:唑来膦酸对牙龈成纤维细胞增殖及凋亡的影响
- Author:
Qingbiao FU
1
;
Caiwen CUI
;
Bin XUAN
;
Yuxuan GUO
;
Chunlin LIU
;
Jian ZHANG
Author Information
1. 辽宁医学院,锦州,121001
- Keywords:
Diphosphonates;
Fibroblasts;
Cell proliferation;
Apoptosis;
Bisphosphonate-related osteonecrosis of the jaw
- From:
Chinese Journal of Stomatology
2015;50(11):667-670
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of different concentrations of zoledronic acid on human gingival fibroblast(HGF) cells proliferation and apoptosis, and to investigate the mechanism of bisphosphonate-related osteonecrosis of the jaw(BRONJ) caused by zoledronic acid.Methods Different concentrations(0, 0.5, 1.0, 5.0, 10.0 μmol/L) of zoledronic acid acted on the HGF.After 24 hours, flow cytometry was used to detect the rate of apoptosis and methyl thiazolyl terazolium(MTF) assay used to observe the proliferation of HGF.The effect of different concentrations of zoledronic acid on cell proliferation was examined by proliferation test on day 2, 4 and 7.Results Flow cytometry showed that when the concentration of zoledronic acid was 0, 0.5, 1.0, 5.0, 10.0 μmol/L, the apoptosis rate was (0.67± 0.50)％, (2.13±0.21)％, (3.27±0.23)％, (4.17±0.35)％, (9.87± 1.79)％ respectively.The difference in HGF cells between control group and zoledronic acid groups was significant(P＜0.05) at three concentrations of zoledronic acid(1.0, 5.0, 10.0 μmol/L).The cell proliferation test showed an concentration-time dependent effect.With increase of concentration and time, the cell proliferation was significantly inhibited.There was significant difference in absorbance value among the different concentration groups(P＜0.05).Conclusions Zoledronic acid can inhibit HGF proliferation and promote its apoptosis.
