Preliminary study on treatment of mandibular osteoradionecrosis by autologous bone marrow mesenchymal stem cells in miniature pigs
10.3760/cma.j.issn.1002-0098.2015.11.007
- VernacularTitle:自体骨髓间充质干细胞治疗放射性骨坏死的动物实验
- Author:
Dianji FANG
1
;
Song LI
;
Lulu ZHAI
;
Zhanyi WANG
;
Yanwei GUO
;
Zhaorong NING
Author Information
1. 上海市闵行区牙病防治所口腔颌面外科
- Keywords:
Osteoradionecrosis;
Myeloid progenitor cells;
Hydroxyapatites;
Tricalcium phosphate
- From:
Chinese Journal of Stomatology
2015;50(11):671-674
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the treatment effect of mandibular osteoradionecrosis(ORN) by autologous bone marrow mesenchymal stem cells(BMMSC) in miniature pigs.Methods Six miniature pigs with mandibular ORN(25 Gy) were used.BMMSC were separated and cultured in vitro and then implanted on to the premolded hydroxyapatite-tricalcium phosphate(HA-TCP).The BMMSC-HA-TCP complexes were implanted into the defective area of mandibular ORN in 4 animals.Implantation of only HA-TCP in 2 animals served as control.Gross observation, spiral CT, coronary CT and histopathologic examination were carried out.Results Fistula disappeared in animals with BMMSC-HA-TCP complexes 3 months after implantation, while fistula was found in controls.Spiral CT analysis showed that cortical bone repair were found 4 months after BMMSC-HA-TCP complexes implantation, while bone damage(cortical and cancellous bone fracture) increased in controls.Histopathologic examination revealed that 4 months later after BMMSC-HA-TCP complexes implantation, new bone formation and bone cells could be observed, but there was a large number of fibrous tissue and no new bone in controls.Conclusions Transplantation of autologous BMMSC with HA-TCP may have therapeutic effect in the treatment of mandibular ORN.
