Effect of endoplasmic reticulum stress on the expression and osteogenic differentiation of periodontal ligament stem cells
10.3760/cma.j.issn.1002-0098.2015.09.010
- VernacularTitle:脂多糖诱导的内质网应激在牙周膜干细胞中的表达及其对成骨分化的影响
- Author:
Peng XUE
1
;
Bei LI
;
Jun TAN
;
Ying AN
;
Yan JIN
;
Qintao WANG
Author Information
1. 第四军医大学口腔医学院牙周科军事口腔医学国家重点实验室
- Keywords:
Periodontal ligament;
Stem cells;
Cell differentiation;
Endoplasmic reticulum stress;
Inflammatory microenvironment
- From:
Chinese Journal of Stomatology
2015;50(9):548-553
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the activity of endoplasmic reticulum stress(ERS) and its effect on osteogenic differentiation of periodontal ligament stem cells(PDLSC) in inflammatory microenvironment.Methods PDLSC were obtained from the primary culture of the human tooth and cloned with limited diluted method.Real-time reverse transcription(RT)-PCR was used to examine the different expression of thapsigargin(TG) treated PDLSC and lipopolysaccharide(LPS) treated PDLSC.Real-time RT-PCR,alizarin red staining and cetyl pyridine chloride quantitative analyze were used to examine the osteogenic differentiation of PDLSC,TG + PDLSC,LPS + PDLSC and LPS + PDLSC + 4-PBA.Results Protein kinase receptorlike endoplasmic reticulum kinase(PERK),glucose regulated protein 78 (GRP78),transcription activation factor 4(ATF4),CCAAT/enhancer-binding protein-homologous protein (CHOP) mRNA expression in group PDLSC+TG in 6 h were respectively 1.49±0.24,2.77±0.60,1.75±0.16,2.16±0.32,which were all greater than that in group PDLSC(P＜0.05).PERK,CHOP mRNA expression reached the peak at 6 h(1.76±0.08,2.31 ±0.17) and were greater than group PDLSC(P＜0.05).ERS could suppress osteogenic differentiation of TG+PDLSC and LPS+PDLSC.The runt-related transcription factor-2 (RUNX2),alkaline phosphatase(ALP),osteocalcin(OCN) mRNA expression of group TG + PDLSC was respectively 0.73±0.06,0.01±0.00,0.20±0.06(P＜0.05).The RUNX2,ALP,OCN mRNA expression of group LPS+PDLSC was respectively 0.80±0.06,0.48±0.05,0.29±0.04(P＜0.05).The RUNX2,ALP,OCN mRNA expression of group PDLSC +TG +4-PBA was respectively 1.10±0.09,0.74±0.05,0.67±0.13,which were greater higher than that of group LPS+PDLSC(P＜O.05).Conclusions ERS was activated in PDLSC and suppressed osteogenic differentiation of PDLSC,which can simulate inflammatory microenvironment in vitro.This effect can be recovered by using ERS inhibitor 4-PBA.
