Comparison of platelet adhesion behavior on pure titanium surfaces modified by different techniques
10.3760/cma.j.issn.1002-0098.2015.09.013
- VernacularTitle:不同改性处理钛表面对血小板黏附行为影响的比较
- Author:
Lu ZHANG
1
;
Chengyun NING
;
Wei TENG
;
Yan WANG
Author Information
1. 中山大学光华口腔医学院·附属口腔医院修复科·广东省口腔医学重点实验室
- Keywords:
Titanium;
Platelet adhesiveness;
Surface modification
- From:
Chinese Journal of Stomatology
2015;50(9):565-569
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the platelet adhesion ability on pure titanium surfaces modified with different techniques.Methods Pure titanium specimens were treated with 5 different surface modification techniques,including machine polish(MP),dual acid-etch(DAE),sandblast-large grit and acidetch(SLA),micro-arc oxidation(MAO) and anodized titania nanotube(TNT).The surface topographies of specimens were observed by scanning electron microscopy(SEM).Chemical compositions,surface roughness and static water contact angle of specimens were detected by energy dispersive spectrometer(EDS),laser scanning confocal microscope(LSCM) and contact angle analyzer respectively.Platelets were cultured on specimen surfaces for 30 min.The amount and viability of adhered platelets adhered were evaluated.Platelet distribution and morphology were observed by LSCM and SEM.Results Surface topographies of the five groups of specimens differed significantly.MP,DAE,SLA and MAO surfaces showed micro-scale topographies,while TNT surfaces showed nano-scale topography with nanotubes at the diameter of (80.46± 0.35) nm.The surface roughness of MAO was the highest among the 5 groups.TNT surfaces demonstrated the lowest roughness as well as the lowest static water contact angle as 13.55°± 0.96°.The amount of platelets adhered on TNT surface was the greatest as (300 729±8 325) platelet/μl and the viability was the highest(A450 value 2.14±0.05).Platelet adhered intensively on TNT surfaces,forming pseudopodia,extending and connecting with each other.Conclusions Surface properties of pure titanium affect platelet adhesion ability.Nano-scale topography can greatly improve platelet adhesion.Increased surface roughness and hydrophilicity can improve platelet adhesion ability.
