Adeno-associated virus mediated T-bet gene transfer into SGC-7901 cell to regulate IFN-gamma production.
- Author:
Gufeng QIU
1
;
Suoying WANG
;
Shengjun WANG
;
Qixiang SHAO
;
Jie MA
;
Ming YANG
;
Xiaopeng XU
;
Chaoming MAO
;
Zhaoliang SU
;
Xinxiang HUANG
;
Huaxi XU
Author Information
1. Department of Immunology, School of Medical Technology, Jiangsu University, Zhenjiang 212013, China.
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Dependovirus;
genetics;
metabolism;
Green Fluorescent Proteins;
biosynthesis;
Humans;
Interferon-gamma;
biosynthesis;
Recombinant Proteins;
biosynthesis;
genetics;
Stomach Neoplasms;
genetics;
metabolism;
T-Box Domain Proteins;
biosynthesis;
genetics;
Transfection
- From:
Journal of Biomedical Engineering
2009;26(3):606-619
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the effect of T-bet on malignant cells, we selected SGC-7901, a kind of human gastric carcinoma cell line, and used gene clone technique and adeno-associated virus (AAV) packing technology, thus obtaining a recombinant rAAV-eGFP-T-bet and T-bet gene-transfected SGC-7901 cells. Then the function of T-bet gene-infected SGC-7901 cells was researched by detecting the levels of IFN-gamma and T-bet production. The results showed: (1) It was verified that rAAV-T-bet's packing was completed; (2) After SGC-7901 cells was transfected by rAAV-eGFP-T-bet, a green fluorescence was found in about 30%-40% SGC-7901s, and the gene of 1670 bp (T-bet) and 388 bp (IFN-gamma) were generated from SGC-7901s cells; (3) The proteins of IFN-gamma and T-bet secreted by SGC-7901 cells were also detected. These reveal that SGC-7901 cell is efficiently infected by rAAV encoding T-bet, which can induce transfected cells to secret IFN-gamma. It may be useful in the researches on cancer immune therapy of transfecting T-bet gene.
