Research on prerequisite for using bovine hemoglobin modified by polyethylene glycol as a red blood cell substitute.
- Author:
Jufeng SUN
1
;
Yunyan LIU
Author Information
1. Pharmaceutical College of Binzhou Medical College, Yantai 264003, China. sjf.xzy@163.com
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Substitutes;
chemical synthesis;
Cattle;
Hemoglobins;
chemistry;
classification;
pharmacology;
Humans;
Hydrogen-Ion Concentration;
Polyethylene Glycols;
chemistry;
classification
- From:
Journal of Biomedical Engineering
2009;26(4):815-819
- CountryChina
- Language:Chinese
-
Abstract:
This paper aims to raise concerns about the modification of bovine hemoglobin with the use of SC-mPEG as modifying reagent. The yield rates of SC-mPEG-BHB under dissimilar conditions are studied, and more important, a series of methods used to characterize the SC-mPEG-BHB mixtures are built. The best reactive system has been confirmed, i.e., at 4 degrees C, pH9; SC-mPEG and BHB are mixed according to the proportion of 60:1 and be submitted to reaction for 2 h.
