Spaceflight alters the gene expression profile of cervical cancer cells.
- Author:
Zhi-Jie ZHANG
1
;
Yong-Qing TONG
;
Jia-Jia WANG
;
Cheng YANG
;
Guo-Hua ZHOU
;
Yue-Hui LI
;
Ping-Li XIE
;
Jin-Yue HU
;
Guan-Cheng LI
Author Information
- Publication Type:Journal Article
- MeSH: Blotting, Northern; methods; Cell Line, Tumor; Cell Proliferation; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Gene Library; Gene Regulatory Networks; Humans; Nucleic Acid Hybridization; methods; Space Flight; Up-Regulation; Uterine Cervical Neoplasms; genetics; pathology
- From:Chinese Journal of Cancer 2011;30(12):842-852
- CountryChina
- Language:English
- Abstract: Our previous study revealed that spaceflight induced biological changes in human cervical carcinoma Caski cells. Here, we report that 48A9 cells, which were subcloned from Caski cells, experienced significant growth suppression and exhibited low tumorigenic ability after spaceflight. To further understand the potential mechanism at the transcriptional level, we compared gene expression between 48A9 cells and ground control Caski cells with suppression subtractive hybridization (SSH) and reverse Northern blotting methods, and analyzed the relative gene network and molecular functions with the Ingenuity Pathways Analysis (IPA) program. We found 5 genes, SUB1, SGEF, MALAT-1, MYL6, and MT-CO2, to be up-regulated and identified 3 new cDNAs, termed B4, B5, and C4, in 48A9 cells. In addition, we also identified the two most significant gene networks to indicate the function of these genes using the IPA program. To our knowledge, our results show for the first time that spaceflight can reduce the growth of tumor cells, and we also provide a new model for oncogenesis study.
