Berberine inhibited apoptosis of human umbilical vein endothelial cells induced by Staphylocoocus aureus: an experimental research.
- Author:
Chuan-yin XIONG
;
Yong-hang FU
;
Han-bin HU
;
Ai-fen BI
;
De-cui PEI
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Berberine; pharmacology; Cell Line; Human Umbilical Vein Endothelial Cells; drug effects; microbiology; pathology; Humans; Staphylococcus aureus
- From: Chinese Journal of Integrated Traditional and Western Medicine 2014;34(6):710-713
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the inhibition of berberine (BBR) against ECV-304 apoptosis induced by Staphylococcus aureus (S. aureus).
METHODSECV-304 cells were pre-treated with 128 microg/mL BBR for 2 h and then S. aureus was added (1:100). The viability of cells was detected by MTT (3-4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The morphological changes were observed by Hoechst 33258 staining. The protection of BBR for infected cells was detected by DNA Ladder.
RESULTSECV-304 cells' viability were not obviously affected by berberine. But S. aureus induced ECV-304 cells' viability could be significantly inhibited by pre-treatment of BBR (P < 0.05). Besides S. aureus-induced ECV-304 apoptosis could be reduced, with significantly lessened apoptotic body and unobvious DNA degradation.
CONCLUSIONBBR could significantly inhibit S. aureus induced ECV-304 apoptosis.