Study on the application and evaluation of methods for gene and antigen detection in plague surveillance program.
- Author:
Rong HAI
1
;
Dong-zheng YU
;
Xian-ming SHI
;
Zhong-bing ZHANG
;
Yong-jiao TANG
;
Peng WANG
;
Lian-xu XIA
;
Shao-zhen WEI
;
Bing XU
;
Ying-xu QIN
;
Zhi-kai ZHANG
;
Guo-xiang SHI
;
Xu DONG-LEI
;
Hong CAI
;
En-min ZHANG
;
Jian-chun WEI
;
Ying-zhi GENG
;
De-hui HUANG
;
Bin ZHAO
;
Li-mao WANG
;
Feng-qin MA
;
Fu HUANG
;
Yue WANG
;
Tao ZHANG
;
Jian-hua ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bacterial Proteins; genetics; immunology; Enzyme-Linked Immunosorbent Assay; Mice; Plague; microbiology; Polymerase Chain Reaction; Yersinia pestis; genetics; immunology; pathogenicity
- From: Chinese Journal of Epidemiology 2007;28(5):426-429
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo apply and evaluate new methods regarding specific gene and antigen detection in plague surveillance program.
METHODS1798 samples from natural foci of plague were tested, using internal quality control multiple-polymerase chain reaction, F1 antigen marked by immuno chromatographic assay and enzyme linked immunosorbent assay. Culture of Yersinia pestis and reverse indirect hemagglutination assay were used as reference diagnostic methods.
RESULTSThe overall positive rate of culture on Yersinia pestis together with gene and antigen detection was 7.34%, showing an 16.81% increase when comparing to 6.28% using Yersinia pestis culture method alone. The rate of coincidence was 97.13%.
CONCLUSIONThe new standard being used for specific gene and antigen detection could increase the positive rate of diagnosis on plague.
