Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from eight province (municipalities) of China.

Ren-fa Zhu; Zhao-long Gong; Chang-yun YE; Zhi-gang Cui; Dong Jin; Lu Ran; Ke XU; Lian-xiu Wang; Wei-wei Chen

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Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from eight province (municipalities) of China.

Author: Ren-fa ZHU ¹ ; Zhao-long GONG ; Chang-yun YE ; Zhi-gang CUI ; Dong JIN ; Lu RAN ; Ke XU ; Lian-xiu WANG ; Wei-wei CHEN
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1. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Journal Article
MeSH: China; Electrophoresis, Gel, Pulsed-Field; Food Microbiology; Listeria monocytogenes; classification; genetics; isolation & purification; Phylogeny
From: Chinese Journal of Epidemiology 2007;28(5):464-467
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo analyse the molecular types of Listeria (L.) monocytogenes, and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis (PFGE) subtyping database, using the international standardized L. monocytogenes-PFGE protocol.
METHODS118 L. monocytogenes strains isolated from 8 provinces or municipalities of China were subtyped according to L. monocytogenes-PFGE protocol.
RESULTS118 strains of L. monocytogenes were divided into 39 subtypes. In the 39 subtypes, 37 strains (31.36%) were GX6A160004 pattern, showing it was the predominant Lm subtype in China.
CONCLUSIONData from molecular typing suggested that the predominant Lm strains were distributed in different regions of China. PulseNet China-Lm database was constructed which was valuable for the molecular subtyping-based surveillance of L. monocytogenes.