Study on the association between estrogen receptor-alpha gene polymorphisms and pubertal skeletal developmental status in China.
- Author:
Li-chen YANG
1
;
Jian-hua PIAO
;
Jie DONG
;
Zhen-wu HUANG
;
Qian ZHANG
;
Guan-Sheng MA
Author Information
- Publication Type:Journal Article
- MeSH: Absorptiometry, Photon; Acid Phosphatase; blood; Adolescent; Alkaline Phosphatase; blood; Bone Density; genetics; physiology; Bone Development; genetics; Child; China; Enzyme-Linked Immunosorbent Assay; Estrogen Receptor alpha; genetics; Female; Genotype; Haplotypes; genetics; Humans; Osteocalcin; blood; Polymerase Chain Reaction; Polymorphism, Genetic; genetics; Polymorphism, Restriction Fragment Length; genetics; Puberty; blood; genetics; Vitamin D; blood
- From: Chinese Journal of Epidemiology 2007;28(6):540-543
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the association between estrogen receptor-alpha (ER-alpha) gene polymorphisms, pubertal bone mineral density (BMD) and bone turnover markers, to better understand the frequencies of ER-a genotypes.
METHODSThe ER-alpha Pvu II and Xba I genotypes were determined by PCR-RFLP in 158 healthy adolescent girls (12-14 years old) while BMD was measured by dual-energy X-ray absorptiometry (DEXA). The bone turnover markers (BAP, BGP, TRACP) and calcium adjust hormone (25-OH-VitD, E2) were detected by ELISA. The relationship among BMD, bone turnover markers, and polymorphisms of ER-a were examined.
RESULTS(1) Although the bone turnover markers had a trend in ER-alpha Pvu II genotypes as pp>PP>Pp but no significant difference was found (P > 0.05). There were no significant differences found among Xba I genotypes and between Px haplotype and non-Px haplotype. (2) No association was noticed between ER-a genotype and BMD at any site of the forearm and the whole body. Compared with non-Px haplotype, the Px haplotype had lower BMD, but there was no significant difference noticed (P > 0.05).
CONCLUSIONER-alpha gene Pvu II and Xba I polymorphism seemed not potentially influence on BMD and bone turnover markers in the pubertal girls in Beijing, suggesting that it might not be the major genetic factor regarding this population.
