Surveillance on Rickettsia in epidemic areas of scrub typhus in Xinyang areas of Henan.
- Author:
Xiu-ping FU
1
;
Jing-shan ZHANG
;
Xiao-jing SHEN
;
Ming-chun LUAN
;
Meng-lei LI
;
Li-juan ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; China; Fluorescent Antibody Technique, Indirect; Humans; Kidney; microbiology; Liver; microbiology; Mice; Orientia tsutsugamushi; classification; genetics; isolation & purification; pathogenicity; Phylogeny; Polymerase Chain Reaction; Rickettsia; classification; genetics; isolation & purification; pathogenicity; Scrub Typhus; epidemiology; microbiology; Spleen; microbiology
- From: Chinese Journal of Epidemiology 2007;28(6):547-549
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo understand the epidemic status of Rickettsia in Xinyang areas of Henan province.
METHODSSamples including liver, spleen, kidney from mouse and chigger mites from Xinyang areas and serum samples were detected by nested-polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA).
RESULTSIn 62 viscus samples from mice organs, the positive rates were 16.13%, 8.06% and 6.45% for Orientia tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. In blood clots samples from mice, the positive rates were 8.06%, 6.45% and 1.61 % for O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae respectively. Three out of 26 mouse serum samples were positive for the predicted fluorexcent intensity O. tsutsugamushi.
CONCLUSIONUsing nested-PCR and IFA methods, O. tsutsugamushi, R. typhii and Spotted fever group rickettsiae were detected in the captured mice living in Xinyang areas of Henan province. Results showed that there were intensive natural reserviors of Rickettsia in Henan province, suggesting that the risk of outbreak of Rickettsia in these areas was high.
