Using direct enzyme linked immunosorbent assay for the detection of IgG antibody on Bartonella henselae among healthy people in Changping, Beijing.
- Author:
Xiao-Ran YANG
1
;
Qi-Yong LIU
;
Bu-Yun CUI
;
Lian-Xiu WANG
;
Zhi-Hui PENG
;
Dong-Sheng REN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Antibodies, Bacterial; immunology; Bartonella henselae; immunology; Enzyme-Linked Immunosorbent Assay; methods; Female; Fluorescent Antibody Technique, Indirect; methods; Humans; Immunoglobulin G; immunology; Male; Middle Aged; Young Adult
- From: Chinese Journal of Epidemiology 2007;28(7):688-691
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect Bartonella henselae IgG antibody among healthy people in Changping, Beijing.
METHODSUsing indirect enzyme linked immunosorbent assay (ELISA) and immunofluorescence antibody assay (IFA) to detect IgG antibody of Bartonella henselae among human beings.
RESULTSThe sensitivity and specificity of ELISA were 70.6% and 91.6% respectively, with the positive predictive value of serological test as 82.2%, and the negative predictive value as 84.9%, based on results of IFA. The positive rate was 34.5% among 357 healthy people on indirect ELISA but was 35.6% among 239 people with IFA.
CONCLUSIONThe results indicated that the indirect ELISA was a very quick, sensitive and available method for detecting Bartonella henselae in human beings, as well as a high positive percent age of Bartonella henselae among the healthy people of Changping Beijing.
