Rapid testing on drug susceptibility of Mycobacterium tuberculosis with nitrate reducrase assay.
- Author:
Zhong-Quan LIU
1
;
Chuan-You LI
;
Xiao-You CHEN
;
Xi CHEN
;
Yu MA
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; pharmacology; Drug Resistance, Bacterial; Enzyme Assays; methods; Humans; Microbial Sensitivity Tests; methods; Mycobacterium tuberculosis; drug effects; pathogenicity; Nitrate Reductase; metabolism; Sputum; microbiology
- From: Chinese Journal of Epidemiology 2007;28(7):697-699
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rapid method for testing drug sussceptibility on Mycobacterium tuberculosis.
METHODSTaking absolute Concentration method for drug susceptibility testing of M. tuberculosis as the "gold standard", we examined the drug-resistant of M. tuberculosis strain with nitrate reducrase assay (NRA) and the drug-resistant of M. tuberculosis germ in sputum with NRA.
RESULTSNRA and absolute concentration method was basically comparable with NRA susceptibility as 96.5% and the specificity was 100%, When comparing with traditional absolute concentration method, NRA could shorten the time about 3 weeks. Using NRA to test the drug-resistant of M. tuberculosis germ in sputum, its susceptibility was more than 66.7% and specificity was 100%, within 10-20 days.
CONCLUSIONNRA could be used as a rapid drug susceptibility testing on M. tuberculosis.
