Proliferation of Cultured Vascular Smooth Muscle Cells(VSMCs) Obtained from Aortas of Insulin Dependent Diabetic Rats.
- Author:
Hyung Joon YOO
1
Author Information
1. Department of Internal Medicine, Hallym University College of Medicine, Seoul, Korea
- Publication Type:Original Article
- Keywords:
Diabetic rat;
Streptozotocin;
Vascular smooth muscle cell;
Proliferati
- MeSH:
Animals;
Aorta*;
Hyperglycemia;
Insulin*;
Muscle, Smooth, Vascular*;
Rats*;
Rats, Sprague-Dawley;
Streptozocin
- From:Journal of the Korean Geriatrics Society
1997;1(1):48-54
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Vascular smooth muscle cells(VS-MCs) from diabetic animals proliferate more than that from non-diabetic ones. The proliferative ability of cultured VSMCs is changed by the kind of experimental animals, experimental technique and so on. In this study, I used VSMCs from streptozotocin-induced insulin dependent diabetic Sprague Dawley rats and examined the characteristics of aortic VSMCs proliferation from these rats. METHODS: Using enzyme method I cultured aortic VSMCs. I compared the proliferation between normal rat VSMCS and diabetic ones. I studied the cell growth of VSMCs from diabetic rats as a function of period after streptozotocin injection. I also examined the effects of hyperglycemia and insulin treatment on the proliferation of cultured VSMCs. RESULTS: Diabetic rats VSMCs proliferated more than non-diabetic ones in streptozotocin-induced insulin dependent diabetic rats. The proliferation of VSMCs was enhanced from 2 weeks after streptozotocin injection. Hyperglycemia enhanced the proliferation and insulin treatment lowered that. CONCLUSIONS: The proliferation of VSMCs was increased in streptozotocin-induced diabetic rats.
