Effects of fosinopril and valsartan on expressions of ICAM-1 and NO in human umbilical vein endothelial cells.
- Author:
Siming GUAN
1
;
Bin WANG
Author Information
- Publication Type:Journal Article
- MeSH: Arteriosclerosis; etiology; prevention & control; Cells, Cultured; Endothelium, Vascular; chemistry; cytology; drug effects; Fosinopril; pharmacology; Humans; Intercellular Adhesion Molecule-1; analysis; Lipoproteins, LDL; pharmacology; Nitric Oxide; analysis; Nitric Oxide Synthase; analysis; Tetrazoles; pharmacology; Umbilical Veins; cytology; drug effects; Valine; analogs & derivatives; pharmacology; Valsartan
- From: Chinese Medical Journal 2003;116(6):923-927
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the effects of fosinopril and valsartan on the expression of intercellular adhesion molecule-1 (ICAM-1) and nitric oxide (NO) induced by oxidized low-density lipoprotein (ox-LDL) in human umbilical vein endothelial cells.
METHODSThe levels of NO, ICAM-1, and nitric oxide synthase (NOS) were determined using the nitrate reductase method, ELISA, immunohistochemical and image analyses.
RESULTSThe ox-LDL can significantly increase the expression of ICAM-1 and inhibit the expression of NO and NOS in a dose-dependent manner. Fosinopril and valsartan can significantly inhibit these roles of ox-LDL. The roles of fosinopril and valsartan were not significantly different.
CONCLUSIONFosinopril and valsartan inhibit oxidized LDL-induced expression of ICAM-1 and increase the expression of NO in human umbilical vein endothelial cells, which is one of the mechanisms of antiatherosclerosis.
