Using a cDNA microarray to study cellular gene expression altered by Mycobacterium tuberculosis.
- Author:
Yongzhong XU
1
;
Jianping XIE
;
Yao LI
;
Jun YUE
;
Jianping CHEN
;
Lijuan CHUNYU
;
Honghai WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; DNA, Complementary; genetics; Gene Expression; Macrophages; Oligonucleotide Array Sequence Analysis; Tuberculosis; genetics
- From: Chinese Medical Journal 2003;116(7):1070-1073
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo examine the global effects of Mycobacterium tuberculosis (M. tuberculosis) infection on macrophages.
METHODSThe gene expression profiling of macrophage U937, in response to infection with M. tuberculosis H(37)R(a), was monitored using a high-density cDNA microarray.
RESULTSM. tuberculosis infection caused 463 differentially expressed genes, of which 366 genes are known genes registered in the Gene Bank. These genes function in various cellular processes including intracellular signalling, cytoskeletal rearrangement, apoptosis, transcriptional regulation, cell surface receptors, cell-mediated immunity as well as a variety of cellular metabolic pathways, and may play key roles in M. tuberculosis infection and intracellular survival.
CONCLUSIONSM. tuberculosis infection alters the expression of host-cell genes, and these genes will provide a foundation for understanding the infection process of M. tuberculosis. The cDNA microarray is a powerful tool for studying pathogen-host cell interaction.
