Human DNA contains sequences homologous to the 5'-non-coding region of hepatits C virus: characterization with restriction endonucleases reveals individual varieties.
- Author:
Reinhard H DENNIN
1
;
Jianer WO
Author Information
- Publication Type:Journal Article
- MeSH: DNA Methylation; Hepacivirus; genetics; Humans; Restriction Mapping; Sequence Homology, Nucleic Acid
- From: Chinese Medical Journal 2003;116(7):1092-1098
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate a 272 base pair section of the 5'-non-coding region of genomic DNA from the peripheral blood monounuclear cells of healthy hepatitis virus C (HCV)-negative human subjects (not patients).
METHODSThis sequence section bears interest because (1) it harbors several potential methylation (Cp-rich) sites, and (2) it represents the largest part of its internal ribosomal entry site. A pre-PCR digestion protocol was established making consistent use of four restriction endonucleases selected for certain features: SmaI, XmaCI, MspI, and HpaII are inhibited if methylation(s) are present at certain cytosines within their cutting sequences.
RESULTSThe suspected HCV-specific sequence was found in the DNA of each subject tested. The pre-PCR digestion assay reveals individual differences in their pattern of methylation, which may be due to possible epigenetic phenomena.
CONCLUSIONSThe results provide formal proof that these HCV-specific sequences are contained in the genomic or extra chromosomal target DNA, and probably belong to a new class of endogenous sequences.
