Effect of curcumin on hippocampal IRS-1 and p-IRS-1 expressions in APP/PS1 double transgenic mice.
- Author:
Hui-Li FENG
1
;
Rui-Sheng LI
;
Hong WANG
;
Ying REN
;
Hai-Yun SUN
;
Jin-Duo YANG
;
Peng-Wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amyloid beta-Protein Precursor; genetics; metabolism; Animals; Curcumin; pharmacology; Hippocampus; drug effects; metabolism; Immunohistochemistry; Insulin Receptor Substrate Proteins; metabolism; Mice; Mice, Transgenic; Reverse Transcriptase Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2013;38(9):1290-1294
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of curcumin on the expressions of insulin receptor substrate-1 (IRS-1) and phosphated insulin receptor substrate-1 (p-IRS-1I) in APP/PS1 double transgenic mice of the AD model.
METHODThree-month-old APP/ PSI double transgenic mice were randomly divided into the model group, the positive rosiglitazone control group and curcumin high (400 mg . kg-1 . d-1), medium (200 mg . kg-1 . d-1) and low (100 mg . kg-1 . d-1) dose groups. The normal group was composed of non-transgenic mice under the same background. After they were orally administered for three months, they were detected with immunohistochemistry, Western blot and RT-PCR.
RESULTAccording to IRS-1 and p-IRS-1 immumohistochemical staining, the expression of IRS-1 positive cells in hippocampus CA1 area in model mice was significantly higher than that of the normal control group (P<0. 01). Compared with the model group, the number of IRS-1 positive cells in hippocampus CA1 area decreased (P <0. 05 or P <0. 01) and the number of p-IRS-1 positive cells in hippocampus CA1 area increased in all of curcumin intervention groups. Western blot results were consistent with IRS-1 and p-IRS-1 protein expressions and immunohistochemistry results. RT-PCR test showed opposite IRS-1 mRNA expression results with immunohistochemistry and Western blot results.
CONCLUSIONCurcumin can recover increased IRS-1 and decreased p-IRS-1 in hippocampus of APP/PS1 double transgenic mice, increase IRS-1 mRNA expression, and improve the insulin-signaling transduction in APP/PS1 double transgenic mice. This suggests that curcumin can regulate the insulin-signaling transduction mechanism and show an anti-AD effect.