Effects of curcumin on expression of PI3K and p-pI3K in hippocampus of AD mice.
- Author:
Hui-Zi DANG
1
;
Rui-Sheng LI
;
Hong WANG
;
Ying REN
;
Hai-Yun SUN
;
Jin-Duo YANG
;
Peng-Wen WANG
Author Information
- Publication Type:Journal Article
- MeSH: Alzheimer Disease; drug therapy; genetics; metabolism; Animals; Curcumin; pharmacology; therapeutic use; Disease Models, Animal; Hippocampus; drug effects; metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; Phosphatidylinositol 3-Kinases; genetics; metabolism; Thiazolidinediones; pharmacology; therapeutic use
- From: China Journal of Chinese Materia Medica 2013;38(9):1295-1299
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of curcumin on the expression of PI3K (phosphatidylinositol-3-kinase, PI3K) and p-P3 K (phosphated phosphatidylinositol-3-kinase, p-PI3K) in the hippocampus of Alzheimer's disease (AD) model (APP/PS1 double transgenic) mice.
METHODA total of 60 three-month-old APP/PS1 double transgenic mice were randomly divided into model group, rosiglitazone group(10 mg . kg-1 . d-1) and curcumin large(400 mg . kg-1 . d-1), medium(200 mg- kg-1 . d-1) and small(100 mg . kg-1 . d-1) dose group. Twelve C57BL/6J mice in the same age and genetic background as APP/PS1 double transgenic mice were used as normal control group. All the 6 groups of mice were intragastrically administered for 3 months. After 3 months, the expression of PI3K and p-PI3K were detected by immunohistochemistry and Western blot.
RESULTThe expression of PI3K and p-PI3K positive cells in hippocampus CA1 region significantly decreased in model group compared with normal control group (P < 0. 05) , while compared with model group, PI3K and p-PI3K positive cells of all the curcumin intervention groups increased to varying degrees in hippocampus CA1 region,especially the middle dose group(P <0. 01). Besides,Western blot results of the curcumin high dose group were also increased obviously (P <0. 05).
CONCLUSIONCurcumin can recover the decreased PI3K and p-PI3K and improve the insulin-signaling transmission in the hippocampus of APP/PS1 double transgenic mice. The mechanism of curcumin maybe by regulating the insulin signal transduction to treat AD.
