Analysis of quality variation and genetic diversity of Desmodium styracifolium from different provenances.
- Author:
Quan YANG
1
;
Ting LU
;
Xue-Yu SANG
;
Quan CHEN
;
Chun-Rong ZHANG
;
Xiao-Min TANG
;
Xuan-Xuan CHENG
;
Shu-Yuan LI
Author Information
- Publication Type:Journal Article
- MeSH: Amplified Fragment Length Polymorphism Analysis; Fabaceae; classification; genetics; Genetic Variation; genetics
- From: China Journal of Chinese Materia Medica 2013;38(9):1344-1348
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the quality variation and genetic diversity of Desmodium styracifolium from different provenances, and lay a foundation for rational exploitation on germplasm resources and fine variety breeding of D. styracifolium.
METHODAmplified fragment length polymorphism (AFLP) markers were developed to analyze genetic diversity in D. styracifolium from 18 resources. NTSYSpc-2. 11F software was used to analyze the similarity among the D. styracifolium germplasms and construct the genetic phylogenetic tree. The schaftoside content in D. styracifolium from different provenances was determined by HPLC.
RESULTA total of 844 fragments were amplified with 8 primers, in which 717 were polymorphic bands, accounting for 84. 27% of the total detected variation. All the specimens from 18 resources could be grouped into 3 clusters by cluster analysis. The schaftoside contents of D. styracifolium germplasms differed significantly, with the highest content in the germplasm from Sanya, Hainan.
CONCLUSIONSignificant quality variation and genetic diversity can be observed among D. styracifolium germplasms. The diverse germplasm resources should be explored and the fine variety should be selected to breed.
