Optimization of extraction process for Baizhu Shaoyao San by multi-index orthogonal experiment.
- Author:
Li-Juan ZHENG
1
;
Kun-Ming QIN
;
Hao CAI
;
Gang CAO
;
Bao-Chang CAI
Author Information
1. Engineering Center for Chinese Medicine Processing under State Ministry of Education, Nanjing University of Chinese Medicine, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Chemical Fractionation;
methods;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
isolation & purification;
Plants, Medicinal;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(10):1504-1509
- CountryChina
- Language:Chinese
-
Abstract:
Baizhu Shaoyao San is a famous traditional Chinese medicine formula, which is efficient to diarrhea clinically. However, there are very few reports for extraction process of Baizhu Shaoyao San at present. In this article, an orthogonal test was conducted, with 10 major components and extractum of Baizhu Shaoyao San as indexes, and data was processed by the compound weight method, in order to detect four factors, including the concentration of ethanol, the dosage of ethanol, the soaking and extracting times, and optimize the extraction process of Baizhu Shaoyao San. The results showed that the optimal extraction process was adding 12-fold ethanol of 70% concentration, soaking for 1 hours, and extracting for twice, 2 hours each time. Among the indexes, the extracting time made significant impact. The optimized method is stable and reliable, and can provide reference for further development and utilization of the formula.
