Study on all-time multi-wavelength fusion fingerprint of Qizhiweitong granules and multi-component quantitative analysis.
- Author:
Dong YAO
1
;
Xian-Sheng MENG
;
Shuai WANG
;
Yong-Rui BAO
;
Ying PAN
;
Ling HAN
Author Information
- Publication Type:Journal Article
- MeSH: Benzoates; analysis; Bridged-Ring Compounds; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; Glucosides; analysis; Glycosides; analysis; Monoterpenes; Quality Control
- From: China Journal of Chinese Materia Medica 2013;38(10):1513-1517
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo control the quality of Qizhiweitong granules with the all-time multi-wavelength fusion fingerprint quantification as the major technique.
METHODAgilent TC-C18 (4.6 mm x 250 mm, 5 microm) chromatographic column was adopted, with 0.02% formic acid water-acetonitrile as the mobile phase for linear gradient elution. The flow rate was 1 mL x min(-1), column temperature was 30 degrees C, and detector wavelength was 230, 254, 283 nm. Matlab was adopted for all-time multiple-wavelength fusion for data in dif format.
RESULTA good relationship was shown for albiflorin in 56.5-452 mg x L(-1) (r = 0.999 8), paeoniflorin in 107-856 mg x L(-1) (r = 0.999 8), licorice glycoside in 73.4-687 mg x L(-1) (r = 0.999 8), naringin in 109-872 mg x L(-1) (r = 0.999 8), neohesperidin in 48.0-384 mg L(-1) (r = 0.999 8), and glycyrrhizic acid in 38.6-308 mg x L(-1) (r = 0.999 8), with recoveries of 0.999 8.
CONCLUSIONThe method is simple, accurate and highly reproducible, and can provide basis for quality control of Qizhiweitong granules.
