Determination of aconitine, hypaconitine and mesaconitine in Shenfu injection.
- Author:
Pan-Pan ZHANG
1
;
Jun-Zhen ZHANG
;
Zhao-Hong WANG
;
Yong-Jiang LU
;
Ye JIANG
Author Information
1. School of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China. zppsjz@126.com
- Publication Type:Journal Article
- MeSH:
Aconitine;
analogs & derivatives;
analysis;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
Mass Spectrometry;
methods;
Quality Control
- From:
China Journal of Chinese Materia Medica
2013;38(10):1521-1525
- CountryChina
- Language:Chinese
-
Abstract:
To establish a method for the content determination of indexes for measuring aconitic compounds contained in Shenfu injection, in order to provide basis for the evaluation of the curative effect of monkshood in Shenfu injection. The sample were purified and enriched with HF-LPME. ACQUITY UPLC BEH C18 column (2.1 mm x 50 mm, 1.7 microm) was adopted and eluted with a gradient program, with acetonitrile-10 mmol x L(-1) NH4HCO3 (pH 10) as the mobile phases. The flow rate was 0.45 mL x min(-1). The content was determined with ESI and MRM. The results showed that aconitine, hypaconitine and mesaconitine showed a good linear relationship, with r > 0.999, within the range of 0.1-100 ng x L(-1). The recoveries were detected to be 100.1%, 97.4%, 97.5%, with RSD being 1.2%, 1.1%, 1.5%, respectively. This method was used to prove the safety of Shenfu injection, and provide scientific basis for correct evaluation of curative effect of monkshood, as well as a reliable, simple and practical means for quality control of monkshood-containing Chinese materia medica preparations.
