Effect of baicalin on expression of TLR4 in RAW264.7 cells infected by ESBLs Escherichia coli.
- Author:
Yun YUN
1
;
Chang-Zhong WANG
;
Li GUI
;
Zhen-Xing LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Escherichia coli; drug effects; physiology; Escherichia coli Infections; drug therapy; genetics; immunology; microbiology; Flavonoids; pharmacology; Gene Expression; drug effects; Humans; Mice; Toll-Like Receptor 4; genetics; immunology
- From: China Journal of Chinese Materia Medica 2013;38(10):1590-1594
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect and mechanism of baicalin against beta-lactamases Escherichia coli (ESBLs E. coli) mediated by toll-like receptor 4 (TLR4) signal transduction pathway.
METHODThe RAW264. 7 cells monolayer pretreated with different concentration of baicalin were inoculated with ESBLs E. coli. The expression of TLR4 mRNA and protein were analyzed by semi-quantitative RT-PCR and Immunofluorescence, respectively. The activity of NF-kappaB was detected by Western blot using total cellular protein. The production of TNF-alpha in supernatant was determined by enzyme linked immunosorbnent assay (ELISA).
RESULTESBLs E. coli significantly up-regulated the expression levels of TLR4 mRNA and protein in a time-dependent manner, induced the activation of NF-KB in RAW264. 7, enhanced the production of TNF-alpha in supernatant. Baicalin down-regulated the expression of TLR4 mRNA and protein, decreased the activation of NF-KB in RAW264. 7 cells and reduced the production of TNF-alpha in supernatant in a dose-dependent manner.
CONCLUSIONBaicailin could inhibit TLR4 signal transduction pathway. The mechanism of baicalin against ESBLs E. coli may be through inhibiting the expression of TLR4 and its signal transduction pathway.
