Simultaneous determination of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in water extract of mixed salviae miltiorrhizae radix et rhizoma and carthami flos by HPLC.
- Author:
Yao LI
1
;
Zheng-Tai GUO
;
Xing-Chu GONG
;
Hai-Bin QU
Author Information
- Publication Type:Journal Article
- MeSH: Benzofurans; analysis; Chalcone; analogs & derivatives; analysis; Chromatography, High Pressure Liquid; methods; Cinnamates; analysis; Depsides; analysis; Lactates; analysis; Quinones; analysis; Rhizome; chemistry; Salvia miltiorrhiza; chemistry
- From: China Journal of Chinese Materia Medica 2013;38(11):1653-1656
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method to determine the contents of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, salvianolic acid B in the water extract of mixed Salviae Miltiorrhizae Radix et Rhizoma and Carthami Flos simultaneously.
METHODThe separation were carried out at 30 degrees C on a ZORBAX Eclipse Plus C18 column (4.6 mm x 100 mm, 1.8 microm) with formic acid-500 mmol x L(-1) ammonium formate-water solution (0.5:10:90) as mobile phase A and acetonitrile-formic acid solution (100: 0.5) as mobile phase B in gradient mode at a flow rate of 0.5 mL x min(-1). Detection wavelengths were 280 nm for danshensu, rosmarinic acid, lithospermic acid, salvianolic acid B, and 380 nm for hydroxysafflor yellow A.
RESULTThe 5 components were separated well with a good linearity (R2 > 0.999 3) in the range of the test concentration. The average recoveries of danshensu, hydroxysafflor yellow A, rosmarinic acid, lithospermic acid, and salvianolic acid B were 99.1%, 102%, 102%, 98.5% and 101%, respectively.
CONCLUSIONThis method is simple, accurate, and repeatable.
