On-line monitoring of extraction process of danhong injection based on near-infrared spectroscopy.
- Author:
Shuang-Yue LIU
1
;
Wen-Long LI
;
Hai-Bin QU
;
Bu-Chang ZHAO
;
Tao ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Carthamus tinctorius; chemistry; Chemistry, Pharmaceutical; methods; standards; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; isolation & purification; Online Systems; Salvia miltiorrhiza; chemistry; Spectroscopy, Near-Infrared; methods
- From: China Journal of Chinese Materia Medica 2013;38(11):1657-1662
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rapid quantitative analysis method for the quality control of Danhong injection extraction using near-infrared (NIR) spectroscopy.
METHODOnline collecting the NIR spectra during the mixed extraction process of Salvia miltiorrhiza and Carthamus tinctorius, partial least squares regression (PLSR) models were developed for the quality indicators rosmarinic acid (RA), salvia acid B (SaB), lithospermic acid (LA), hydroxysafflor yellow A (HSYA) and solid content (SSC), with HPLC and weight-loss method as reference methods.
RESULTThe correlation coefficients of the cross validation for RA, SaB, LA, HSYA and SSC were 0.909 3, 0.915 2, 0.901 9, 0.747 7 and 0.931 4, respectively. And the root mean square errors of cross validation (RMSECV) were 0.012 1, 0.251, 0.017 7, 0.038 1 g x L(-1) and 0.359%, respectively.
CONCLUSIONIn this study, NIR spectroscopy was successfully applied to achieve the real-time determination of the contents of RA, SaB, LA and SSC, while the performance of the HSYA calibration model needed to be improved.
