Application of ultraviolet spectroscopy for rapid analysis in extraction process of danhong injection.
- Author:
Bin-Jun YAN
1
;
Shuang-Yue LIU
;
Zheng-Tai GUO
;
Shi-Chao HUANG
;
Hai-Bin QU
Author Information
1. Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China. ybinjun@126.com
- Publication Type:Journal Article
- MeSH:
Chemistry, Pharmaceutical;
standards;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal;
analysis;
isolation & purification;
Quality Control;
Salvia miltiorrhiza;
chemistry;
Spectrophotometry, Ultraviolet;
methods
- From:
China Journal of Chinese Materia Medica
2013;38(11):1676-1678
- CountryChina
- Language:Chinese
-
Abstract:
In this work, a rapid analysis method basing on ultraviolet spectroscopy was established for the determination of danshensu, protocatechuic aldehyde, rosmarinci acid, lithospermic acid and salvianolic acid B in the extraction process of Danhong injection. In the extraction process of Danshen and Honghua crude drugs, 44 extraction solution samples were collected and the contents of the five components were determined by HPLC analysis. The ultraviolet spectra of the samples were collected. Partial least square regression was used to establish the multivariate calibration models between the ultraviolet spectra and the contents of the five components. The results showed that the established models could predict the contents of the five components in the extraction solution accurately. The ultraviolet spectroscopy method established in this work can be used for rapid analysis of the intermediates of Danhong injection, which may be applied for the quality control in the manufacturing process.
