Isolation and characteristics of Panax ginseng autotoxin-degrading bacterial strains.
- Author:
Dong-Yue ZHAO
1
;
Yong LI
;
Wan-Long DING
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China. zhaodongyue1987@126.com
- Publication Type:Journal Article
- MeSH:
Bacteria;
classification;
genetics;
isolation & purification;
metabolism;
DNA, Bacterial;
genetics;
DNA, Ribosomal;
genetics;
Molecular Sequence Data;
Panax;
chemistry;
growth & development;
metabolism;
microbiology;
Phylogeny;
RNA, Ribosomal, 16S;
genetics;
Soil;
chemistry;
Soil Microbiology;
Toxins, Biological;
metabolism
- From:
China Journal of Chinese Materia Medica
2013;38(11):1703-1706
- CountryChina
- Language:Chinese
-
Abstract:
In this study, traditional plate culturing method was used to isolate autotoxin-degrading microbial strains, and which were then identified by 16S rDNA homological analysis and morphological characteristics. Furthermore, the growth and autotoxin-degrading efficiency of them were analyzed by liquid culturing method and GC-MS to illustrate their autotoxin-degradation characteristics. As a result, five bacterial strains having autotoxin-degrading activity were isolated from 6-years ginseng nonrhizospheric soil successfully, and which can growth successfully by taking autotoxins added artificially as carbon source in liquid culturing condition. Results indicated that it was feasible to isolate autotoxin-degrading bacteria from ginseng nonrhizospheric soil, and the isolated bacterial strains can be used to degrade autotoxins in soils once planted Panax ginseng.
