Analysis on chemical components from water extract of paeoniae radix alba by high performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry.
- Author:
Dong-Dong SUN
1
;
Xiao-Fang XU
;
Jiu-Cheng CUI
;
Xiao-Mei SONG
;
Xiang LI
Author Information
1. College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China. sun_21373@163.com
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
isolation & purification;
Molecular Structure;
Paeonia;
chemistry;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods
- From:
China Journal of Chinese Materia Medica
2013;38(11):1760-1765
- CountryChina
- Language:Chinese
-
Abstract:
This experiment was performed to establish a qualitative analysis on chemical composition in water extract of Paeoniae Radix Alba by HPLC-ESI-Q-TOF-MS. The analysis was conducted on a C18 (Hanbon Lichrospher, 4.6 mm x 250 mm, 5 microm) column with methanol-0.1% formic acid as the mobile phase for gradient elution; ESI ion source was used for mass spectra, and data were collected in both positive and negative modes. The results showed that eleven compounds from water extract of Paeoniae Radix Alba had been identified by analyzing positive and negative ion mass data including element composition and by comparing with data from literatures. Since efficient separation of HPLC and the high sensitive detection of MS was used, this experiment, it will provide evidences for elucidation of the effective substance in the water extract of Paeoniae Radix Alba.
